Characterization of vegetal plate cilia during echinoid gastrulation.
Ettinger, Chelsea R.
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The objective of the present study was to characterize the growth, differentiation, lifecycle, and orientation of vegetal plate cilia during Echinoid gastrulation. Focus was placed on the role these cilia might play in PCP signaling during early embryonic development. Cilia at the vegetal plate were quantified for orientation, length, and retraction rate in order to better characterize the cilia in this region. In addition,methodologies for the immunofluorescence labeling of basal bodies were developed in order to better characterize the establishment of planar cell polarity at the vegetal plate during gastrulation. The data show that the archenteron cilia in both Strongylocentrotus purpuratus and Dendraster excentricus are motile. In addition, the results suggest that planar polarity establishment at the vegetal plate is a gradual process, while the length transition from lateral to vegetal cilia is abrupt. Finally, the data suggest that two distinct rates of ciliary retraction occur at the vegetal plate.
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