Patterns of ciliary retraction in echinoid embryos.
Sholi, Adam N.
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In this study sea urchin embryos were used to investigate how cilia are disassembled and to investigate the role that cilia play in cell division. A retraction mechanism was proposed in which the ciliary axoneme is entirely withdrawn into the cell cytoplasm before it is completely disassembled. This mechanism may be mediated by a cortically-localized, plus-end directed motor protein. To determine biochemical distributions of the axoneme and mitotic spindle, a ratiometric imaging technique was developed. Analysis of ratiometric images suggests that the axoneme is destabilized by a deacetylase enzyme which may function to regulate the levels of alpha-tubulin and acetylated tubulin present in the mitotic spindle. The use of ratiometric imaging will be useful for future experiments that investigate the role of other post-translational modifications to tubulin. This study reports the identification of two distinct retraction modes which appear to be independent of cilia subtype or length.
Description:Thesis--Departmental honors in Biochemistry.MIME type:application/pdfFile Size:2.804Mb