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dc.contributor.authorRist, Michael P.
dc.date.accessioned2014-10-13T19:07:10Z
dc.date.available2014-10-13T19:07:10Z
dc.date.issued2014
dc.identifier.otherW Thesis 1457
dc.identifier.urihttp://hdl.handle.net/11040/23884
dc.descriptionii, 46 leaves.en_US
dc.descriptionBibliography: leaves 38-42.
dc.description.abstract"Methylmercury (MeHg) is an environmental toxin that targets the nervous system but can cause an array of effects on other systems as well. The main source of exposure is through fish consumption, because MeHg bioaccumulates in fresh water and marine food chains. MeHg has a high affinity for selenium and sulfur in the body, which assists in its solubility as well as its toxicity. MeHg has been shown to inhibited redox regulatory enzymes such as glutathione peroxidase (GPx). Inhibition of this enzyme leads to toxic levels of reactive oxygen species (ROS) which can damage DNA, proteins and lipids inside of the cell. Neutrophils, cells of the innate immune response, utilize these ROS to degrade pathogens. These cells utilize GPx to regulate ROS from causing cell damage. Protocols, assays and analytical methods were developed and modified to measure GPx activity and amount of lipid peroxidation within cell cultures. Embryonic chick brain cells were used to develop and modify methods. These cells showed decreases in GPx activity but not lipid oxidation. The neutrophil cell culture did not produce enough signals to measure, suggesting a possible interference within the neutrophil matrix. The data from this study suggests that cell culture can be used to analyze direct exposure of MeHg but the methods might need to be altered for every different cell that is being tested."en_US
dc.description.tableofcontentsMercury as an environmental pollutant -- Mechanisms of MeHg toxicity -- Neutrophils and the respiratory burst -- The effects of MeHg on the immune system -- Experimental design -- Cell culture -- MeHg exposure and incubation -- Protein concentration -- Glutathione peroxidase analytical method -- TBARS analytical method -- Cell culture and MeHg toxicity assays -- Chick embryo brain cell results -- Neutrophil culture results.
dc.language.isoen_USen_US
dc.publisherWheaton College (Norton, Mass.)en_US
dc.subjectUndergraduate research.
dc.subjectUndergraduate thesis.
dc.subject.lcshMethylmercury -- Bioaccumulation.
dc.subject.lcshMethylmercury -- Toxicology -- Analysis.
dc.subject.lcshMethylmercury -- Physiological effect.
dc.subject.lcshMethylmercury -- Health aspects.
dc.subject.lcshMethylmercury -- Research.
dc.subject.lcshEnvironmental toxicology.
dc.subject.lcshNeutrophils -- Immunology.
dc.titleEffects of MeHg on ROS regulation in neutrophil cell cultureen_US
dc.typeThesisen_US


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