• Login
    View Item 
    •   Digital Repository Home
    • Works by Students
    • Senior Honors Theses
    • Neuroscience
    • View Item
    •   Digital Repository Home
    • Works by Students
    • Senior Honors Theses
    • Neuroscience
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Ciliogenesis during zebra fish heart regeneration.

    Thumbnail
    View/Open
    Thesis (2.477Mb)
    Date
    2015
    Author
    Pope, Hans William.
    Metadata
    Show full item record
    Abstract
    Cardiac ischemic injury, a heart attack, occurs when blood flow through the coronary arteries is obstructed. As a result in humans, necrotic tissue is replaced by hard, inelastic scar tissue that impairs the heart’s ability to function. The human heart possesses little to no regenerative capacity on its own to recovery from ischemic injury. Zebrafish, however, are capable of robust cardiac regeneration after injury to the heart. Through a series of remarkable heart-localized events that mirror embryonic development, the zebrafish can replace damaged or lost heart tissue within a month. We noticed the similarity between the regeneration and development of cardiac tissue may implicate cilia are involved during regeneration. Cilia are microtubule-based organelles that serve important sensory functions during embryonic development and organ homeostasis. It is well established that sensory cilia regulate Hedgehog and Wnt signaling pathways to aid in differentiating tissues during embryogenesis. Recent studies provide evidence that cilia are present during cardiogenesis and are necessary for proper cardiomyocyte differentiation and cardiac morphology. Thus, we hypothesized cilia may play similar roles during zebrafish heart regeneration. In the current study, we tested heart tissue for the presence of cilia using immunofluorescent confocal microscopy. Our preliminary data suggest cilia are abundant in regenerating cardiac tissue 7 days after removing a portion of the ventricular apex. In addition, we began to implement the surgical procedures necessary to injure the zebrafish ventricle at Wheaton College.
    URI
    http://hdl.handle.net/11040/24312
    Collections
    • Neuroscience [6]
    • File:Hans Pope_Senior Honors Thesis_Defense Draft_Ver05_2015 (Final Draft) .pdf
      Description:Thesis
      MIME type:application/pdf
      File Size:2.477Mb

    Browse

    All of Digital RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    Wheaton College Massachusetts
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV