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dc.contributor.authorCressey, Lauren
dc.date.accessioned2019-04-15T14:21:57Z
dc.date.available2019-04-15T14:21:57Z
dc.date.issued2018
dc.identifier.otherW Thesis 1538
dc.identifier.urihttp://hdl.handle.net/11040/24545
dc.descriptionii, 36 leaves: illustrations.
dc.descriptionIncludes bibliographical references: leaves 30-36.
dc.description.abstractEstrogen is a female sex hormone that is important in the development of secondary sex characteristics in females and promotes breast cancer growth and metastasis of estrogen receptor (ER)-positive breast cancer cells. Environmental estrogens, such as metalloestrogens, have also been shown to promote breast cancer growth. Methylmercury (MeHg) is a metalloestrogen whose role in breast cancer growth and progression has not been elucidated. MeHg is of interest because it is the main form of mercury in humans and aquatic life, as it accumulates through the aquatic food chain. To test whether MeHg promotes the growth of breast cancer cells in vivo, a Danio rerio embryo model was used. MCF-7 ER positive breast cancer cells were transplanted into a 2-day post fertilization embryos (dpf). Tumor growth and metastasis were observed on 7 dpf using fluorescent imagery. We hypothesized that ER-postivie breast cancer cells treated with MeHg would have increased tumor growth and more metastases compared to untreated embryos. MeHg treated embryos showed higher percentages of embryos with cancer, tumor count, and tumor area compared to untreated embryos. From these results, we conclude that MeHg may be acting as a metalloestrogen to promote the growth and progression of ER-positive breast cancer in xenografted Danio rerio embryos.en_US
dc.description.tableofcontentsAbstract – Chapter 1: Introduction – 1.1 Breast cancer – 1.2 Estrogen and ER – 1.3 Estrogen receptor in breast cancer – 1.4 Xenoestrogens and metalloestrogens – 1.5 Methylmercury – 1.6 Zebrafish – 1.7 ER in Zebrafish – 1.8 Hypothesis – Chapter 2: Materials and methods – 2.1 Cell lines and primary culture conditions – 2.2 Zebrafish embryo care – 2.3 MCF-7/GFP cell xenotransplantation – 2.3 MeHg treatment – 2.4 Zebrafish imaging – 2.5 Statistical analysis – 2.6 Tumor analysis – Chapter 3: Results – 3.1 20 nM MeHg treatment of danio rerio embryos significantly impacts survival rate – 3.1 Methylmercury promotes an increase in tumor growth and metastasis – Chapter 4: Discussion – References
dc.language.isoen_USen_US
dc.publisherWheaton College (MA).en_US
dc.subjectUndergraduate research.en_US
dc.subjectUndergraduate thesis.en_US
dc.subject.lcshMethylmercury -- Health aspects.
dc.subject.lcshZebra danio -- Research.
dc.subject.lcshBreast -- Cancer -- Environmental aspects.
dc.subject.lcshChemical carcinogenesis.
dc.subject.lcshCancer invasiveness.
dc.titleMethylmercury promotes the growth and progression of breast cancer in danio rerio embryos.en_US
dc.title.alternativeSpine label: Methylmercury promotes the growth and progression of breast cancer.en_US
dc.typeThesisen_US


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