Patterns of ciliary retraction in echinoid embryos.

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Authors
Sholi, Adam N.
Issue Date
2014
Type
Thesis
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en_US
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Undergraduate research. , Undergraduate thesis.
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Abstract
In this study sea urchin embryos were used to investigate how cilia are disassembled and to investigate the role that cilia play in cell division. A retraction mechanism was proposed in which the ciliary axoneme is entirely withdrawn into the cell cytoplasm before it is completely disassembled. This mechanism may be mediated by a cortically-localized, plus-end directed motor protein. To determine biochemical distributions of the axoneme and mitotic spindle, a ratiometric imaging technique was developed. Analysis of ratiometric images suggests that the axoneme is destabilized by a deacetylase enzyme which may function to regulate the levels of alpha-tubulin and acetylated tubulin present in the mitotic spindle. The use of ratiometric imaging will be useful for future experiments that investigate the role of other post-translational modifications to tubulin. This study reports the identification of two distinct retraction modes which appear to be independent of cilia subtype or length.
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74 leaves : illustrations.
Bibliography: leaves 71-74.
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Wheaton College (Norton, Mass.)
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